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pyromark assay design 2.0 software  (Qiagen)


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    Qiagen pyromark assay design 2.0 software
    Pyromark Assay Design 2.0 Software, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pyromark+assay+design+software/pyromark+assay+design+2+0+software/pmc12266127-354-8-13
    Average 90 stars, based on 1 article reviews
    pyromark assay design 2.0 software - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Pyromark Assay:

    Article Title: PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation.
    Article Snippet: The low expression of period circadian regulator 3 (PER3) in head and neck squamous cell carcinoma is closely correlated with tumor size and invasion depth.. Hypoxia-inducible factor 1 subunit alpha (HIF-1α) regulates epithelial-mesenchymal transition (EMT) transcription factors, activates EMT, and promotes tumor metastasis.. Here, we investigated the role and molecular mechanism of PER3 in regulating HIF-1α and metastasis in oral squamous cell carcinoma (OSCC) by using bioinformatics analyses and in vitro and in vivo experiments.

    Article Title: Profiling of Circulating Cell-free DNA Methylation Patterns Identifies Aberrant Methylated CTBP1 Promotor Sites for Prediction of Alzheimer’s Disease
    Article Snippet: The differentially methylated candidate genes were validated using pyrosequencing on a cohort of blood samples (17 AD and 17 HC). .. Pyrosequencing assay was designed with the PyroMark Assay Design software (version 2.0, Qiagen). ..

    Article Title: Molecular responses of chicken embryos to maternal heat stress through DNA methylation and gene expression: a pilot study.
    Article Snippet: The pyrosequencing was performed using PyroMark Q24 (Qiagen). .. All the primers (forward, reverse, and sequencing primers) were designed with the PyroMark Assay Design software (Version 2.0.1.15, Qiagen) using the assay type ‘Methylation Analysis’ (CpG) (Table S6). .. The PCR contained 2 μl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 μl of buffer + 0.05 μl of Taq Polymerase (PCRBIO Classic Taq, Eurobio), 2.5 μl of dNTP (2 mM, Promega), 1 μl of each primer (10 μM), and 5.95 μl of water.

    Article Title:
    Article Snippet: .. Selection of the primers were generated with the PyroMark Assay Design software (Version 2.0; Qiagen) and PCR products were first tested with PCR protocol to determine the suitability of the PCR products for the sequence. .. Pyrosequence is performed using a PyroMark Q96 MD system (Qiagen).

    Article Title: Ageing limits stemness and tumorigenesis by reprogramming iron homeostasis.
    Article Snippet: Xueqian Zhuang, Qing Wang, Simon Joost, Alexander Ferrena, David T. Humphreys, Zhuxuan Li, Melissa Blum, Klavdija Krause, Selena Ding, Yuna Landais, Yingqian Zhan, Yang Zhao, Ronan Chaligne, Joo-Hyeon Lee, Sebastian E. Carrasco, Umeshkumar K. Bhanot, Richard P. Koche, Matthew J. Bott, Pekka Katajisto, Yadira M. Soto-Feliciano, Thomas Pisanic, Tiffany Thomas, Deyou Zheng, Emily S. Wong & Tuomas Tammela1 ✉

    Article Title: Molecular responses of chicken embryos to maternal heat stress through DNA methylation and gene expression: a pilot study
    Article Snippet: The pyrosequencing was performed using PyroMark Q24 (Qiagen). .. All the primers (forward, reverse, and sequencing primers) were designed with the PyroMark Assay Design software (Version 2.0.1.15, Qiagen) using the assay type ‘Methylation Analysis’ (CpG) ( ). .. The PCR contained 2 μl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 μl of buffer + 0.05 μl of Taq Polymerase (PCRBIO Classic Taq, Eurobio), 2.5 μl of dNTP (2 mM, Promega), 1 μl of each primer (10 μM), and 5.95 μl of water.

    Article Title: Relationships between serotonin 1A receptor DNA methylation, self-reported history of childhood abuse and gray matter volume in major depression.
    Article Snippet: Background: Early life adversity is a risk factor for psychopathology and is associated with epigenetic alterations in the 5-HT1A receptor gene promoter.. The 5-HT1A receptor mediates neurotrophic effects, which could affect brain structure and function.. We examined relationships between self-reported early childhood abuse, 5-HT1A receptor promoter DNA methylation, and gray matter volume (GMV) in Major Depressive Disorder (MDD).

    Article Title: EPM2 AIP1 immunohistochemistry as a surrogate of promoter methylation analysis in endometrial carcinoma.
    Article Snippet: .. Subsequently, the promoter regions between positions − 248 and − 178 from the transcription start site (TSS) and containing 8 CpG sites were amplified by PCR using a specific primer set developed by PyroMark Assay Design software (Qiagen GmbH, Germany). .. The generated Amplicon was sequenced using Pyromark Q24 (Qiagen GmbH, Germany), and the methylation status of the MLH1 promoter was determined by analysis of the sequence with PyroMark CpG software (Qiagen GmbH, Germany).

    Software:

    Article Title: PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation.
    Article Snippet: The low expression of period circadian regulator 3 (PER3) in head and neck squamous cell carcinoma is closely correlated with tumor size and invasion depth.. Hypoxia-inducible factor 1 subunit alpha (HIF-1α) regulates epithelial-mesenchymal transition (EMT) transcription factors, activates EMT, and promotes tumor metastasis.. Here, we investigated the role and molecular mechanism of PER3 in regulating HIF-1α and metastasis in oral squamous cell carcinoma (OSCC) by using bioinformatics analyses and in vitro and in vivo experiments.

    Article Title: Profiling of Circulating Cell-free DNA Methylation Patterns Identifies Aberrant Methylated CTBP1 Promotor Sites for Prediction of Alzheimer’s Disease
    Article Snippet: The differentially methylated candidate genes were validated using pyrosequencing on a cohort of blood samples (17 AD and 17 HC). .. Pyrosequencing assay was designed with the PyroMark Assay Design software (version 2.0, Qiagen). ..

    Article Title: Molecular responses of chicken embryos to maternal heat stress through DNA methylation and gene expression: a pilot study.
    Article Snippet: The pyrosequencing was performed using PyroMark Q24 (Qiagen). .. All the primers (forward, reverse, and sequencing primers) were designed with the PyroMark Assay Design software (Version 2.0.1.15, Qiagen) using the assay type ‘Methylation Analysis’ (CpG) (Table S6). .. The PCR contained 2 μl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 μl of buffer + 0.05 μl of Taq Polymerase (PCRBIO Classic Taq, Eurobio), 2.5 μl of dNTP (2 mM, Promega), 1 μl of each primer (10 μM), and 5.95 μl of water.

    Article Title:
    Article Snippet: .. Selection of the primers were generated with the PyroMark Assay Design software (Version 2.0; Qiagen) and PCR products were first tested with PCR protocol to determine the suitability of the PCR products for the sequence. .. Pyrosequence is performed using a PyroMark Q96 MD system (Qiagen).

    Article Title: Ageing limits stemness and tumorigenesis by reprogramming iron homeostasis.
    Article Snippet: Xueqian Zhuang, Qing Wang, Simon Joost, Alexander Ferrena, David T. Humphreys, Zhuxuan Li, Melissa Blum, Klavdija Krause, Selena Ding, Yuna Landais, Yingqian Zhan, Yang Zhao, Ronan Chaligne, Joo-Hyeon Lee, Sebastian E. Carrasco, Umeshkumar K. Bhanot, Richard P. Koche, Matthew J. Bott, Pekka Katajisto, Yadira M. Soto-Feliciano, Thomas Pisanic, Tiffany Thomas, Deyou Zheng, Emily S. Wong & Tuomas Tammela1 ✉

    Article Title: Molecular responses of chicken embryos to maternal heat stress through DNA methylation and gene expression: a pilot study
    Article Snippet: The pyrosequencing was performed using PyroMark Q24 (Qiagen). .. All the primers (forward, reverse, and sequencing primers) were designed with the PyroMark Assay Design software (Version 2.0.1.15, Qiagen) using the assay type ‘Methylation Analysis’ (CpG) ( ). .. The PCR contained 2 μl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 μl of buffer + 0.05 μl of Taq Polymerase (PCRBIO Classic Taq, Eurobio), 2.5 μl of dNTP (2 mM, Promega), 1 μl of each primer (10 μM), and 5.95 μl of water.

    Article Title: Relationships between serotonin 1A receptor DNA methylation, self-reported history of childhood abuse and gray matter volume in major depression.
    Article Snippet: Background: Early life adversity is a risk factor for psychopathology and is associated with epigenetic alterations in the 5-HT1A receptor gene promoter.. The 5-HT1A receptor mediates neurotrophic effects, which could affect brain structure and function.. We examined relationships between self-reported early childhood abuse, 5-HT1A receptor promoter DNA methylation, and gray matter volume (GMV) in Major Depressive Disorder (MDD).

    Article Title: EPM2 AIP1 immunohistochemistry as a surrogate of promoter methylation analysis in endometrial carcinoma.
    Article Snippet: .. Subsequently, the promoter regions between positions − 248 and − 178 from the transcription start site (TSS) and containing 8 CpG sites were amplified by PCR using a specific primer set developed by PyroMark Assay Design software (Qiagen GmbH, Germany). .. The generated Amplicon was sequenced using Pyromark Q24 (Qiagen GmbH, Germany), and the methylation status of the MLH1 promoter was determined by analysis of the sequence with PyroMark CpG software (Qiagen GmbH, Germany).

    Sequencing:

    Article Title: PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation.
    Article Snippet: The low expression of period circadian regulator 3 (PER3) in head and neck squamous cell carcinoma is closely correlated with tumor size and invasion depth.. Hypoxia-inducible factor 1 subunit alpha (HIF-1α) regulates epithelial-mesenchymal transition (EMT) transcription factors, activates EMT, and promotes tumor metastasis.. Here, we investigated the role and molecular mechanism of PER3 in regulating HIF-1α and metastasis in oral squamous cell carcinoma (OSCC) by using bioinformatics analyses and in vitro and in vivo experiments.

    Article Title: Molecular responses of chicken embryos to maternal heat stress through DNA methylation and gene expression: a pilot study.
    Article Snippet: The pyrosequencing was performed using PyroMark Q24 (Qiagen). .. All the primers (forward, reverse, and sequencing primers) were designed with the PyroMark Assay Design software (Version 2.0.1.15, Qiagen) using the assay type ‘Methylation Analysis’ (CpG) (Table S6). .. The PCR contained 2 μl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 μl of buffer + 0.05 μl of Taq Polymerase (PCRBIO Classic Taq, Eurobio), 2.5 μl of dNTP (2 mM, Promega), 1 μl of each primer (10 μM), and 5.95 μl of water.

    Article Title:
    Article Snippet: .. Selection of the primers were generated with the PyroMark Assay Design software (Version 2.0; Qiagen) and PCR products were first tested with PCR protocol to determine the suitability of the PCR products for the sequence. .. Pyrosequence is performed using a PyroMark Q96 MD system (Qiagen).

    Article Title: Ageing limits stemness and tumorigenesis by reprogramming iron homeostasis.
    Article Snippet: Xueqian Zhuang, Qing Wang, Simon Joost, Alexander Ferrena, David T. Humphreys, Zhuxuan Li, Melissa Blum, Klavdija Krause, Selena Ding, Yuna Landais, Yingqian Zhan, Yang Zhao, Ronan Chaligne, Joo-Hyeon Lee, Sebastian E. Carrasco, Umeshkumar K. Bhanot, Richard P. Koche, Matthew J. Bott, Pekka Katajisto, Yadira M. Soto-Feliciano, Thomas Pisanic, Tiffany Thomas, Deyou Zheng, Emily S. Wong & Tuomas Tammela1 ✉

    Article Title: Molecular responses of chicken embryos to maternal heat stress through DNA methylation and gene expression: a pilot study
    Article Snippet: The pyrosequencing was performed using PyroMark Q24 (Qiagen). .. All the primers (forward, reverse, and sequencing primers) were designed with the PyroMark Assay Design software (Version 2.0.1.15, Qiagen) using the assay type ‘Methylation Analysis’ (CpG) ( ). .. The PCR contained 2 μl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 μl of buffer + 0.05 μl of Taq Polymerase (PCRBIO Classic Taq, Eurobio), 2.5 μl of dNTP (2 mM, Promega), 1 μl of each primer (10 μM), and 5.95 μl of water.

    Methylation:

    Article Title: Molecular responses of chicken embryos to maternal heat stress through DNA methylation and gene expression: a pilot study.
    Article Snippet: The pyrosequencing was performed using PyroMark Q24 (Qiagen). .. All the primers (forward, reverse, and sequencing primers) were designed with the PyroMark Assay Design software (Version 2.0.1.15, Qiagen) using the assay type ‘Methylation Analysis’ (CpG) (Table S6). .. The PCR contained 2 μl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 μl of buffer + 0.05 μl of Taq Polymerase (PCRBIO Classic Taq, Eurobio), 2.5 μl of dNTP (2 mM, Promega), 1 μl of each primer (10 μM), and 5.95 μl of water.

    Article Title: Molecular responses of chicken embryos to maternal heat stress through DNA methylation and gene expression: a pilot study
    Article Snippet: The pyrosequencing was performed using PyroMark Q24 (Qiagen). .. All the primers (forward, reverse, and sequencing primers) were designed with the PyroMark Assay Design software (Version 2.0.1.15, Qiagen) using the assay type ‘Methylation Analysis’ (CpG) ( ). .. The PCR contained 2 μl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 μl of buffer + 0.05 μl of Taq Polymerase (PCRBIO Classic Taq, Eurobio), 2.5 μl of dNTP (2 mM, Promega), 1 μl of each primer (10 μM), and 5.95 μl of water.

    Selection:

    Article Title:
    Article Snippet: .. Selection of the primers were generated with the PyroMark Assay Design software (Version 2.0; Qiagen) and PCR products were first tested with PCR protocol to determine the suitability of the PCR products for the sequence. .. Pyrosequence is performed using a PyroMark Q96 MD system (Qiagen).

    Generated:

    Article Title:
    Article Snippet: .. Selection of the primers were generated with the PyroMark Assay Design software (Version 2.0; Qiagen) and PCR products were first tested with PCR protocol to determine the suitability of the PCR products for the sequence. .. Pyrosequence is performed using a PyroMark Q96 MD system (Qiagen).

    Polymerase Chain Reaction:

    Article Title:
    Article Snippet: .. Selection of the primers were generated with the PyroMark Assay Design software (Version 2.0; Qiagen) and PCR products were first tested with PCR protocol to determine the suitability of the PCR products for the sequence. .. Pyrosequence is performed using a PyroMark Q96 MD system (Qiagen).

    Article Title: EPM2 AIP1 immunohistochemistry as a surrogate of promoter methylation analysis in endometrial carcinoma.
    Article Snippet: .. Subsequently, the promoter regions between positions − 248 and − 178 from the transcription start site (TSS) and containing 8 CpG sites were amplified by PCR using a specific primer set developed by PyroMark Assay Design software (Qiagen GmbH, Germany). .. The generated Amplicon was sequenced using Pyromark Q24 (Qiagen GmbH, Germany), and the methylation status of the MLH1 promoter was determined by analysis of the sequence with PyroMark CpG software (Qiagen GmbH, Germany).

    Amplification:

    Article Title: Ageing limits stemness and tumorigenesis by reprogramming iron homeostasis.
    Article Snippet: Xueqian Zhuang, Qing Wang, Simon Joost, Alexander Ferrena, David T. Humphreys, Zhuxuan Li, Melissa Blum, Klavdija Krause, Selena Ding, Yuna Landais, Yingqian Zhan, Yang Zhao, Ronan Chaligne, Joo-Hyeon Lee, Sebastian E. Carrasco, Umeshkumar K. Bhanot, Richard P. Koche, Matthew J. Bott, Pekka Katajisto, Yadira M. Soto-Feliciano, Thomas Pisanic, Tiffany Thomas, Deyou Zheng, Emily S. Wong & Tuomas Tammela1 ✉

    Article Title: EPM2 AIP1 immunohistochemistry as a surrogate of promoter methylation analysis in endometrial carcinoma.
    Article Snippet: .. Subsequently, the promoter regions between positions − 248 and − 178 from the transcription start site (TSS) and containing 8 CpG sites were amplified by PCR using a specific primer set developed by PyroMark Assay Design software (Qiagen GmbH, Germany). .. The generated Amplicon was sequenced using Pyromark Q24 (Qiagen GmbH, Germany), and the methylation status of the MLH1 promoter was determined by analysis of the sequence with PyroMark CpG software (Qiagen GmbH, Germany).



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